Abstract: Two new flavonoid glycosides were isolated from the aerial parts of Nervilia fordii by various chromatographies such as D101 macroporous resin, ODS and preparative HPLC chromatographic techniques. Their structures were elucidated as rhamnocitrin-3-O-β-glucopyranosyl-4'-O-β-galactosyl-(1→3)-glucopyranoside (1) and 7, 3'-di-O-methylquercetin-4'-O-β-galactosyl-(1→3)-β-glucopyranosyl-3-O-β-glucopyranoside (2) on the basis of extensive spectroscopic analyses, including 1D-, 2D-NMR, HR-ESI-MS and analytical hydrolysis.