UHPLC-MS法鉴定大鼠灌服开心散后血中远志糖酯类化合物及其代谢产物

Identification of polygala oligosaccharide esters and their metabolites in rat plasma after oral administration of ethanol extract of Kai Xin San by UHPLC-MS

摘要: 本研究利用超高效液相色谱-飞行时间质谱联用技术（UHPLC-TOF/MS），借助液质联用工作站Masslynx V4.1分析软件，结合现有文献及相关数据库，鉴定了开心散60%乙醇提取物（KXS-60% E）中20个远志糖酯类成分；在此基础上，进一步指认并鉴定了灌服KXS-60% E后大鼠血中的14个远志糖酯类化合物及4个来源于远志糖酯类成分的代谢产物。以上研究结果表明，远志糖酯类化合物及其代谢产物是KXS-60% E的主要血中移行成分，原形及代谢产物可能是开心散防治AD的潜在药效物质基础。

Abstract: Using a UHPLC-TOF/MS method combined with software of Masslynx V4.1 and database in the literatures, a total of 20 polygala oligosaccharide esters (POEs) were identified in 60% ethanol extract of Kai Xin San (KXS-60%E). Furthermore, 14 POEs as well as 4 POE-metabolites were identified in rat plasma. The results revealed that POEs and POE-metabolites which were identified in rat plasma, were key components in KXS-60%E and the potential bioactive compounds for KXS action in the treatment of AD.