基于luciferin-luciferase体系的肼生物发光探针的合成、体外评价及活体成像

Synthesis, in vitro evaluation and in vivo imaging of a highly selective hydrazine bioluminescent probe based on luciferin-luciferase system

摘要: 本文设计合成了一个用于可视化分析检测肼（N2H4）的生物发光探针（bioluminescent probe for hydrazine，BPH），并完成对其光学响应性能和活体成像的评价。本文所描述的肼生物发光探针（BPH）是通过在光学报告基团萤火虫荧光素（firefly luciferin）的适当修饰位点合理设计引入肼的特异性识别基团（乙酰基）以达到检测肼的目的，并且该探针以高选择性和高灵敏度等显著优点成功应用于活体水平对肼的可视化分析。实验结果表明，无论是在复杂的自然环境中还是在动物体内，本研究所发展的生物发光探针BPH是一种具有高创新性和广泛应用价值的检测肼的方法。

Abstract: A highly sensitive and selective bioluminescent probe for hydrazine (BPH) was designed, synthe sized and evaluated for detection of hydrazine in vitro and in vivo. BPH was designed to include a specific recogni tion group (acetyl) of hydrazine at an appropriate modification site of the optical reporter hydroxyluciferin (Dluciferin), which showed excellent performance both in selectivity and sensitivity to hydrazine. The results showed that the bioluminescent probe BPH developed in this study is an innovative and widely applicable tool for detecting hydrazine in complex natural environment or in animals.