Abstract: N6-methyladenosine (m⁶A) modification is one of the most common modifications of eukaryotic mRNA, and has become a hotspot in the field of life sciences in recent years. m⁶A modification is dynamically reversible in mammalian cells and regulated by m⁶A methyltransferase (writers), demethylase (erasers), and "reader" proteins. m⁶A can regulate various biological processes of mRNA such as RNA splicing, nuclear export, protein translation and degradation. Recent studies indicated that m⁶A is important for the initiation and development of cancer. The present review summarized biological functions of m⁶A on mRNA and discussed its roles in cell proliferation, migration, invasion, cell mentalism, and angiogenesis. Further, the m⁶A can regulate the development of various cancers including acute myelocytic leukemia (AML), breast, liver and colorectal cancer. Nowadays, the inhibitors of m⁶A related enzymes including fat-mass and obesity-associated protein and AlkB homolog 5 are being developed. We further discussed the potential values of m⁶A and its related targets on cancer therapy and treatment.