醋制延胡索中一个新的原小檗碱型二聚体生物碱

A new dimeric dehydrocorydaline alkaloid from vinegar-prepared Corydalis yanhusuo

摘要: 运用大孔吸附树脂、硅胶柱色谱、反相中压柱色谱、半制备高效液相色谱等分离纯化技术，从醋制延胡索（Corydalis yanhusuo W.T.Wang）中乙酸乙酯部位分离获得1个新的通过亚甲二氧基方式连接的原小檗碱型对称二聚体生物碱，通过UV、IR、HR-ESI-MS、1D NMR和2D NMR等多种波谱分析方法确定其结构，并命名为双去氢紫堇碱A（bidehydrocorydaline A）（1）。化合物1可显著抑制脂多糖（LPS）诱导小鼠巨噬细胞RAW 264.7释放NO，IC₅₀值为2.33 ±0.57 μmol·L^-1。

Abstract: Bidehydrocorydaline A (1), a new dimeric alkaloid of proberberine connected by a methylenedioxy group, was isolated from the vinegar-prepared Corydalis yanhusuo by various chromatographic methods, including column chromatography over macroporous adsorption resin and silica gel, reverse-phase MPLC, and semi-preparative HPLC. Its structure was determined by spectroscopic methods, including UV, IR, HR-ESI-MS, 1D and 2D NMR. Bidehydrocorydaline A (1) showed potent inhibitory activity against LPS-induced NO production in RAW 264.7 macrophages, with an IC₅₀ value of 2.33 ±0.57 μmol·L^-1.