赵玉升, 田雨, 李伟洋, 陈玉民, 曹天佑, 赵亚芳, 李郁茹, 屈会化, 赵琰. 葶苈子炭新型纳米类成分的发现及其止血机制研究J. 药学学报, 2022,57(2): 492-499. doi: 10.16438/j.0513-4870.2021-0824
引用本文: 赵玉升, 田雨, 李伟洋, 陈玉民, 曹天佑, 赵亚芳, 李郁茹, 屈会化, 赵琰. 葶苈子炭新型纳米类成分的发现及其止血机制研究J. 药学学报, 2022,57(2): 492-499. doi: 10.16438/j.0513-4870.2021-0824
ZHAO Yu-sheng, TIAN Yu, LI Wei-yang, CHEN Yu-min, CAO Tian-you, ZHAO Ya-fang, LI Yu-ru, QU Hui-hua, ZHAO Yan. Nano-components derived from Descurainiae Semen Carbonisatum and its hemostatic mechanismJ. Acta Pharmaceutica Sinica, 2022,57(2): 492-499. doi: 10.16438/j.0513-4870.2021-0824
Citation: ZHAO Yu-sheng, TIAN Yu, LI Wei-yang, CHEN Yu-min, CAO Tian-you, ZHAO Ya-fang, LI Yu-ru, QU Hui-hua, ZHAO Yan. Nano-components derived from Descurainiae Semen Carbonisatum and its hemostatic mechanismJ. Acta Pharmaceutica Sinica, 2022,57(2): 492-499. doi: 10.16438/j.0513-4870.2021-0824

葶苈子炭新型纳米类成分的发现及其止血机制研究

Nano-components derived from Descurainiae Semen Carbonisatum and its hemostatic mechanism

  • 摘要: 在葶苈子炭中提取分离出一种新型纳米类成分,并通过药理实验研究其止血机制。利用马弗炉制备250℃、300℃和350℃的葶苈子炭,通过提取分离方法获得各温度下的葶苈子炭透析液,利用低分辨透射电镜(TEM)和高分辨透射电镜(HR-TEM)对该纳米类成分进行表征,利用紫外光谱(UV-Vis)、荧光光谱(FL)和红外光谱(FTIR)测定其光学特征及官能团信息,利用小鼠肝脏出血实验评价其止血效果,进一步检测凝血4项参数和血小板(PLT)的数量来研究其机制。所有动物实验均符合北京中医药大学伦理委员会的规定。TEM结果显示,葶苈子炭袋内透析液中存在着一种新型纳米类成分,并命名为葶苈子炭纳米类成分(DSC-NCs)。小鼠肝脏出血实验结果表明,250℃、300℃和350℃制备的DSC-NCs都能够降低小鼠肝脏出血时间,其中,以350℃制备的DSC-NCs效果最好。此外,各温度制备的DSC-NCs也能够不同程度地降低凝血酶原时间(PT)值、升高纤维蛋白原(FIB)值和PLT值。综上,DSC-NCs有一定的止血效果,这可能与激活外源凝血系统、提高FIB值及PLT含量有关,这为探索出血性疾病治疗药物提供了新的研究方向,也为DSC-NCs在医学领域的潜在应用提供了新的视角。

     

    Abstract: A novel sort of nano-component was extricated and isolated from Descurainiae Semen Carbonisatum (DSC), and its hemostatic component was considered through pharmacological experiments. A muffle furnace was used to prepare DSC at 250℃, 300℃ and 350℃, and the DSC dialysate at each temperature was obtained by the extraction and separation method. Low-resolution transmission electron microscopy (TEM) and high-resolution transmission electron microscopy (HR-TEM) were utilized to characterize the nano-components. Ultraviolet spectroscopy (UV-Vis), fluorescence spectroscopy (FL) and infrared spectroscopy (FTIR) were utilized to measure its optical characteristics and functional group information. The anti-hemorrhagic effects were evaluated by liver bleeding tests and the related hemostatic mechanisms of the obtained nano-components were further assessed by detecting blood coagulation and PLT quantity to discuss the hemostasis mechanism. The experiments complied with the Animal Ethics Committee of Beijing University of Chinese Medicine. TEM results showed that there was a novel type of nano-component in the DSC dialysate bag, which was named DSC nano-components (DSC-NCs). The experimental results of liver bleeding in mice showed that DSC-NCs prepared at 250℃, 300℃, and 350℃ could reduce the bleeding time of mice liver. Among them, DSC-NCs prepared at 350℃ had the best effect. In addition, DSC-NCs prepared at various temperatures can also reduce the prothrombin time (PT) value, increase the fibrinogen (FIB) value and the platelet (PLT) value to varying degrees. DSC-NCs have a certain hemostatic effect, which may be related to the activation of the exogenous coagulation system, the increase of FIB value and the increase of platelet content. This provides a new research direction for exploring the treatment of bleeding diseases, and provides a new perspective for the potential application of DSC-NCs in the medical field.

     

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