广藿香油中1个新的广藿香烷型倍半萜及其抗炎活性

A new patchoulane-type sesquiterpenoid from patchouli oil and its anti-inflammatory activity

摘要: 采用硅胶柱色谱、中压液相色谱、半制备高效液相色谱等方法对广藿香油进行分离纯化, 并根据IR、HR-ESI-MS、NMR及X-单晶衍射等手段鉴定化合物的结构, 得到1个新的7, 10∶1, 5 型广藿香烷型倍半萜, 鉴定为(-)-(3S, 4R, 5R, 7R, 10R)-7, 10∶1, 5patchoul-1(2)-en-3, 4-diol (1)。对化合物1进行了细胞抗炎活性实验, 结果表明, 化合物1对LPS诱导的RAW264.7细胞释放NO具有抑制作用。

Abstract: A new 7, 10∶1, 5-patchoulane-type sesquiterpenoid was isolated and purified by silica gel column chromatography, medium pressure liquid chromatography, and semi-preparative high performance liquid chromatography. Based on IR, HR-ESI-MS, NMR, and X-single crystal diffraction data analyses, compound 1 was determined to be (-)-(3S, 4R, 5R, 7R, 10R)-7, 10∶1, 5patchoul-1(2)-en-3, 4-diol. It showed an inhibitory effect on LPS-induced NO production in RAW264.7 cells.