火把花内生真菌<i>Trichoderma atroviride</i> B7生物碱类成分研究

火把花内生真菌Trichoderma atroviride B7生物碱类成分研究

摘要: 采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱和高效液相色谱等方法从火把花内生真菌Trichoderma atroviride B7固体发酵提取物中分离得到9个生物碱类化合物。利用核磁共振谱、高分辨质谱等方法将其分别鉴定为: atroviridanol (1)、3-oxo-3-(2-phenylethyl) amino-propanoic acid (2)、N-(2′-phenylethyl)-acetamide (3)、neoechinulin A (4)、echinulin (5)、gancidin W (6)、N-isobutyl-3-methylbutanamide (7)、5-acetamido-1-pentanol (8) 和N-2-methylpropyl-2-methylbutenamide (9)。其中, 化合物1为新化合物, 化合物2~9首次从木霉属真菌中分离得到。

Abstract: Nine compounds were isolated from the crude extract of the solid culture of endophyte Trichoderma atroviride B7 of Colquhounia coccinea var. mollis by silica gel column chromatography, Sephadex LH-20 gel column chromatography, and HPLC. They were identified as atroviridanol (1), 3-oxo-3-(2-phenylethyl) amino-propanoic acid (2), N-(2′-phenylethyl)-acetamide (3), neoechinulin A (4), echinulin (5), gancidin W (6), N-isobutyl-3-methylbutanamide (7), 5-acetamido-1-pentanol (8), and N-2-methylpropyl-2-methylbutenamide (9) by NMR, HR-MS, and so on. Among them, compound 1 is a new compound, and compounds 2-9 are firstly isolated from Trichoderma spp.