藿香中的两个新乌苏烷型三萜

Two new ursane triterpenoids from Agastache rugosa (Fisch. et.Mey.) O. Kuntze

摘要: 采用硅胶、MCI、ODS柱色谱和高效制备液相色谱等方法, 从藿香80%乙醇提取物中分离得到2个新乌苏烷型三萜和12个已知化合物。通过质谱、核磁共振和ECD等波谱学方法鉴定新化合物的结构分别为2α, 3α-dihydroxy-24-nor-urs-4(23), 12(13)-dien-28-oic acid (1)和2α, 3α-dihydroxy-24-nor-urs-4(23), 12(13), 20(30)-trien-28-oic acid (2), 命名为agasursacid A和agasursacid B。对分离得到的部分化合物测定了抗柯萨奇病毒B3型(CVB3)活性, 化合物3、4、6、8对柯萨奇病毒B3型有抑制活性, IC₅₀值分别为4.77、1.59、11.11、25.87 μmol·L^-1。

Abstract: Two new ursane triterpenoids along with twelve known compounds were isolated from 80% ethanol extract of Agastache rugosa (Fisch. et. Mey.) O. Kuntze by using silica gel column, MCI column, ODS column and HPLC. The structures of the new compounds were identified as 2α, 3α-dihydroxy-24-nor-urs-4(23), 12(13)-dien-28-oic acid (1) and 2α, 3α-dihydroxy-24-nor-urs-4(23), 12(13), 20(30) -trien-28-oic acid (2) by HR-ESI-MS, NMR and ECD spectral data, named agasursacid A and agasursacid B. In addition, compounds 3, 4, 6, 8 showed anti-coxsackievirus B3 (CVB3) activities with a IC₅₀ as 4.77, 1.59, 11.11 and 25.87 μmol·L^-1, resepectively.