少棘巨蜈蚣中二个新含氮芳香苷的分离鉴定

Isolation and identification of two nitrogen-containing aromatic glycosides from Scolopendra subspinipes mutilans

摘要: 通过大孔树脂、MCI gel CHP 20P、ODS-A-HG、Sephadex LH-20等色谱方法反复柱层析, 并结合TLC和反相HPLC色谱方法, 从药用动物少棘巨蜈蚣醇提物的乙酸乙酯部位中分离得到3个苷类和2个肽类化合物。借助UV、1D/2D NMR和HR-ESI-MS等现代波谱技术分别鉴定为colosides A和B (1和2)、吲哚-3-甲酰基-β-D-吡喃葡萄糖苷(3)、橙黄胡椒酰胺(4) 和环(L-苯丙-L-缬) (5)。其中1和2为新化合物、3和4为首次从蜈蚣中分到的化合物。

Abstract: Using column chromatography methods including the macroporous adsorbent resin, MCI gel CHP 20P, ODS-A-HG, Sephadex LH-20, combined with chromatographic separation methods such as TLC and reversed-phase HPLC, three glycosides (1-3) and two peptides including two new compounds were isolated from the ethanol extract of arthropod Scolopendra subspinipes mutilans. Their structures were identified as colosides A and B (1 and 2), 3, 4-dihydroxyquinoline-4-O-β-D-glucopyranoside (3), aurantiamide (4), cyclo (L-phe-L-val) (5) by UV, NMR and HR-ESI-MS spectroscopic techniques.