茯苓中一个新孕甾烷类化合物

A new pregnane steroid from Poria cocos

摘要: 采用ODS、MCI gel CHP20以及硅胶等柱色谱技术并结合半制备液相对茯苓95%乙醇提物进行分离纯化, 运用NMR、MS、IR、计算NMR等方法, 对分离得到的化合物进行结构鉴定。从茯苓中共分离得到7个化合物, 分别鉴定为20S-2β, 3α, 15α, 19, 20-羟基-孕甾-7-烯(1)、去氢齿孔酸乙酰酯(2)、齿孔酸乙酰酯(3)、去氢齿孔酸(4)、齿孔酸(5)、去氢茯苓酸(6)、茯苓酸(7)。化合物1为新孕甾烷类化合物。

Abstract: The 95% ethanol extract of Poria cocos was separated and purified by ODS, MCI gel CHP20 and silica gel column chromatography combined with the semi-preparative HPLC. The chemical structures of the isolated compounds were identified by NMR, MS, IR, and calculated NMR methods. Seven compounds were isolated from Poria cocos and identified as 20S-2β, 3α, 15α, 19, 20-hydroxy-pregnane-7-ene (1), dehydroeburicoic acid monoacetate (2), eburicoic acid acetate (3), dehydroeburicoic acid (4), eburicoic acid (5), dehydropachymic acid (6), pachymic acid (7). Compound 1 is a new pregnane steroid.