Abstract: Nineteen compounds were isolated from the ethyl acetate fraction of the stems and leaves of Salvia miltiorrhiza Bge by using various column chromatographic methods, such as silica gel, Sephadex LH-20 and semi-preparative HPLC. According to their physiochemical properties and spectral data, the structures were further identified as 3′,4′-dihydroxy-4-(3-methoxy-3-oxopropyl)-1,1′-biphenyl-3-carboxylic acid (1), 2-hydroxy-5-(2′-hydroxybenzyl) benzoic acid (2), dehydrozingerone (3), 3,4-dihydroxy benzalacetone (4), benzyl alcohol-β-D-glucopyranoside (5), dehydrololiolide (6), rel-5-(3S,8S-dihydroxy-1R,5S-dimethyl-7-oxa-6-oxobicyclo3,2,1-oct-8-yl)-3-methyl-2Z,4E-pentadienoic acid (7), 6-hydroxy-3-(2-hydroxyethyl)-2,4,4-trimethylcy clohexa-2,5-dien-1-one (8), scopoletin (9), esculetin (10), dihydrotanshinone Ⅰ (11), 2′-deoxythymidine (12), quercetin 3-glucoside (13), sutchuenmedin A (14), 2′′-O-rhamnosylicariside-Ⅱ (15), 2,2′-oxybis(1,4-di-tert-butylbenzene) (16), 1H-indole-3-carbaldehyde (17), 3-(2-oxopropyl)-3-hydroxyind-olin-2-one (18), dysodensiol E (19). Compound 1 was a new biphenyl lignan, named as 3′,4′-dihydroxy-4-(3-methoxy-3-oxopropyl)-1,1′-biphenyl-3-carboxylic acid. Compound 2 was a new natural product and compounds 3-8 and 4-19 were firstly isolated from S. miltiorrhiza. MTT method was used to screen the anti-hepatoma activity of all the compounds, and none of them showed obvious effect.