罗焕亮, 郭勇, 崔堂兵, 代建国, 张俊松, 许柏球. 真菌诱导子对胡桐悬浮培养细胞产生红厚壳素的影响J. 药学学报, 2004, 39(4): 305-308.
引用本文: 罗焕亮, 郭勇, 崔堂兵, 代建国, 张俊松, 许柏球. 真菌诱导子对胡桐悬浮培养细胞产生红厚壳素的影响J. 药学学报, 2004, 39(4): 305-308.
LUO Huan-liang, GUO Yong, CUI Tang-bing, DAI Jian-guo, ZHANG Jun-song, XU Bai-qiu. Effects of fungal elicitor on inophyllums production in suspension cultured cells of Calophyllum inophyllum L.J. Acta Pharmaceutica Sinica, 2004, 39(4): 305-308.
Citation: LUO Huan-liang, GUO Yong, CUI Tang-bing, DAI Jian-guo, ZHANG Jun-song, XU Bai-qiu. Effects of fungal elicitor on inophyllums production in suspension cultured cells of Calophyllum inophyllum L.J. Acta Pharmaceutica Sinica, 2004, 39(4): 305-308.

真菌诱导子对胡桐悬浮培养细胞产生红厚壳素的影响

Effects of fungal elicitor on inophyllums production in suspension cultured cells of Calophyllum inophyllum L.

  • 摘要: 目的探讨真菌诱导子对胡桐悬浮培养细胞产生红厚壳素的影响。方法通过对胡桐叶斑病病菌的分离培养,制成真菌诱导子补加到胡桐细胞悬浮培养基中,考察不同浓度、不同加入时间对胡桐细胞生物量及红厚壳素产量的影响。结果S-I菌株诱导胡桐CR2细胞合成红厚壳素的最佳浓度为60 mg GE·L-1,诱导子在细胞生长静止期初期(即培养d 18)加入对红厚壳素产量影响最大,可使产量提高27%,并促进了红厚壳素向胞外分泌。结论 壳多孢菌的添加能有效提高胡桐细胞悬浮培养体系中红厚壳素的产量。

     

    Abstract: AimTo investigate the effects of fungal elicitors on inophyllums production in suspension cultured cell of Calophyllum inophyllum Linn. MethodsThe pathogen of leaf spot disease of C.inophyllum L. was isolated and prepared as fungal elicitor. The fungal elicitor was added to the medium with different concentrations and culture period. Their effects on biomass and inophyllums content of the suspension of cultured cells were studied. ResultsThe optimum effects of S-I fungal elicitor concentrations on inophyllums content was 60 mg GE·L-1. Adding the fungi elicitor into the cell suspension culture system at stationary phase (being cultured for 18 days) resulted in a highest inophyllum content of 59.174 mg·L-1 at the 3rd day with 27% higher than control. Fungal elicitor treatment promoted the inophyllums accumulation in medium. ConclusionAdding the Stagonospora curtisii (Berk.) Sacc.to the medium was effective approaches to enhance inophyllums yield in the suspension of C.inophyllum L culture cell.

     

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