Abstract: A HPLC method, in comparison with UV method, for determination of piroxicam (PIX) contents in suppositories made of semisynthetic fatty base (FBS) or PEG base (PEGS) is described. The method provides good reproducibility, favorable stability and yet high senstivity. No significant differences were found between the HPLC and UV method by Student t test (P>0.05).According to the results obtained from each of 5 batches of FBS and PEGS, the mean contents of PIX in FBS and in PEGS being 99.7±0.9% and 100.3±0.7% by HPLC method, and 99.7±0.9% and 100.2±0.6% by UV method, it was concluded that the two methods were equally satisfactory for the determination of the contents of PIX in suppositories. Furthermore, the good separation characteristic makes the HPLC method described here not only applicable to suppository containing single remedy such as piroxicam, but also applicable to those containing compounded nonsteroidal anti-inflammatory drugs (NSADs) or other NSADs drug alone.The chromatography was carried out on a model YSB-Ⅱ liquid chromatography fitted with a reversed-phase YWG-C₍₁₈₎ (10μm) column and equipped with a 254 nm UV detector. The mobile phase was a mixture of methanol—0.2 mol/L ammonium acetate (1:1 v/v) with a flow rate of 1 ml/min. Sodium sulfamethazin was used as an internal standard.