周 晖 邱立朋 闫笑笑 李 琳 李 翔 王 璐 刘 美 王东凯. 奥沙利铂纳米结构脂质载体的制备及表征J. 药学学报, 2010,45(9): 1177-1182.
引用本文: 周 晖 邱立朋 闫笑笑 李 琳 李 翔 王 璐 刘 美 王东凯. 奥沙利铂纳米结构脂质载体的制备及表征J. 药学学报, 2010,45(9): 1177-1182.
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ZHOU Hui, Qiu-Li-Peng, Yan-Xiao-Xiao, Li- Lin, Li- Xiang, Wang- Lu, Liu- Mei, Wang-Dong-Kai. Preparation and characterization of oxaliplatin-loaded nanostructured lipid carriersJ. 药学学报, 2010,45(9): 1177-1182.
Citation: ZHOU Hui, Qiu-Li-Peng, Yan-Xiao-Xiao, Li- Lin, Li- Xiang, Wang- Lu, Liu- Mei, Wang-Dong-Kai. Preparation and characterization of oxaliplatin-loaded nanostructured lipid carriersJ. 药学学报, 2010,45(9): 1177-1182.
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奥沙利铂纳米结构脂质载体的制备及表征

Preparation and characterization of oxaliplatin-loaded nanostructured lipid carriers

    摘要
  • 摘要:

    采用乳化超声法制备奥沙利铂纳米结构脂质载体 (oxaliplatin-loaded nanostuctured lipid carriers, OP-NLC), 通过正交试验筛选出最优处方。运用激光粒度仪、zeta电位仪、透射电镜 (transmission electron  microscope, TEM)、差示扫描量热法 (differential scanning calorimetry, DSC)、X衍射 (X-ray diffraction, XRD) 以及高效液相色谱法 (high performance liquid chromatography, HPLC) 等考察其理化性质。结果显示, 优化条件下制备的OP-NLC多为类球形粒子, 平均粒径 (111 ± 20) nm, zeta电位为 (−27.4 ± 13.1) mV, 包封率 (encapsulation efficiency, EE) 为 (77.4 ± 2.5) %, 药物含量为 (0.8 ± 1.5) mg·mL−1, DSC及XRD表明药物以非结晶状分散于纳米粒中。体外释放实验表明, OP-NLC在pH 4.5的缓冲液中释药前期有突释现象, 后期则具有明显的缓释特征。

     

    Abstract:

    Oxaliplatin-loaded nanostuctured lipid carriers (OP-NLC) were prepared by ultrasonic emulsification method.  And its optimal prescription was selected by orthogonal design.  The laser light scattering technique, zeta potential analyzer, TEM, DSC, XRD and HPLC were employed to study the physicochemical parameters of OP-NLC, which displayed in terms of particle size, zeta potential, crystalline, drug loading and encapsulation efficiency.  The results showed that OP-NLC had an average diameter of (111 ± 20) nm, zeta  potential of (−27.4 ± 13.1) mV, encapsulation efficiency of (77.4 ± 2.5) % and drug content of (0.8 ± 1.5) mg·mL−1.  TEM, DSC and XRD indicated that OP-NLC was spherical and the drug was dispersed as nanoparticles by means of non-crystalline.  The in vitro release test showed that the drug could be sustained-released from NLC in buffer solution (pH 4.5) after a burst release in initial phase.

     

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