Abstract: AimTo study the synthetic method and antibacterial activity of amino-heterocyclic compounds coupled oxime-ether group. MethodsThe treatment of 4-amino-3-methyl-5-mercapto-s-triazole (3) with β-chlorophenyl-propanone to form amino-s-triazole sulfanylphenyl-propanone (4) sequentially followed by oximation with hydroxyl-amine to produce the oximes (5) and etherification with various oxadiazole chloromethanes (6a-j) to yield the title compounds (1a-j). The in vitro antibacterial activities of all newly synthesized compounds were tested against Gram positive bacteria and Gram negative bacteria with the standard 2-fold agar dilution method. ResultsTwelve new compounds including two intermediates were synthesized and their structures were confirmed by IR,¹H NMR, MS and elemental analyses. The ten title compounds exhibited the potential antibacterial activities in vitro. ConclusionTheses compounds should be optimized.