藏波罗花根中两个酚苷的分离纯化、结构鉴定和抗氧化活性研究(英文)
Isolation, purification and structure identification of two phenolic glycosides from the roots of Incarvillea younghusbandii Sprague and their antioxidant activities
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摘要:
利用生物活性跟踪法, 通过硅胶吸附柱色谱、反相C18柱色谱和反相制备型HPLC, 从藏波罗花的根中筛选到两个化合物。通过ESI-MS、GC-MS、1D-和2D-NMR进行结构鉴定, 确定为acteoside (1) 和isoacteoside (2)。1和2的•OH清除活性与苯甲酸接近, 1和2的•OH及O•2–清除活性均远远高于维生素C, 1和2的肝组织LPO抑制活性也高于BHT或维生素C, 1和2在保护红细胞免受氧化损伤的活性也高于维生素C。在0.015 6至0.500 0 mg·mL−1浓度范围, 1和2的•OH清除活性与浓度成正相关关系。在0.001 9至0.250 0 mg·mL−1浓度范围, 随着浓度的增大, 1和2的肝组织LPO抑制活性不断增强; 但在0.250 0至1.000 0 mg·mL−1浓度范围, 随着浓度的增大, LPO抑制活性反而有轻微的下降。
Abstract:Using a bioassay-guided fractionation technique, two compounds were isolated from the roots of Incarvillea younghusbandii Sprague through silica gel, reverse-phase C18 column chromatography and reverse- phase HPLC. Their structures were identified as acteoside (1) and isoacteoside (2) by ESI-MS, GC-MS, 1D- and 2D-NMR. 1 and 2 showed •OH scavenging capacity similar with benzoic acid, higher O•2– (or •OH) scavenging capacity than ascorbic acid, far higher hepatic LPO inhibitory activities than 2, 6-di-tert-butyl-4- methylphenol (BHT) or ascorbic acid, and more powerful effect on protecting erythrocytes from oxidative damage than ascorbic acid. The •OH scavenging capacity was positively proportional to the concentrations of 1 and 2 ranging from 0.015 6 to 0.500 0 mg·mL−1. The hepatic LPO inhibitory activities increased with the increasing concentrations of 1 and 2 from 0.001 9 to 0.250 0 mg·mL−1, but decreased slightly with the increasing concentration from 0.250 0 to 1.000 0 mg·mL−1.
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