HPLC法测定番荔枝科植物中番荔素含量

DETERMINATION OF ANNONACEOUS ACETOGENINS IN ANNONACEAE PLANTS BY HPLC

摘要: 目的　建立番荔枝科植物中抗肿瘤活性成分番荔素的HPLC含量测定方法。方法　Squamostatin-B (1) ,squamocin (2 )和annonin-VI (3)为标准品。色谱柱:反相C₁₈柱;流动相:甲醇-水(90∶10 ) ;流速:1.0mL·min^-1 ;检测波长:220nm。结果　进样量在2.3- 13.8μg有良好的线性关系。(1) ,(2)和(3)的回收率分别为100.3% ,100.3%和100.0 %。结论　本法快速、简便、灵敏和分离度好,适用于番荔枝科植物中抗肿瘤活性成分番荔素的含量测定。

Abstract: AIM To develop a method for analysis of antitumor annonaceous acetogenins in Annonaceae plants by HPLC. METHODS Squamostatin-B (1), squamocin (2) and annonin-VI (3) were used as standard substances. Chromatography column was a Rp-18; the mobile phase was methanol-water (90∶10); the flow rate was 1.0 mL·min^-1 and the detecting wavelength was 220 nm. RESULTS A linear range was obtained from 2.3 to 13.8 μg with a good correlation. The recoveries of (1), (2) and (3) were 100.3%, 100.3% and 100.0%, respectively. CONCLUSION This method was developed for the analysis of acetogenins by HPLC for the first time. The method is rapid, accurate and suitable for the analysis of the antitumor acetogenins in Annonaceae plants.