HPLC-ELSD法测定浙贝母中主要生物碱的含量

Determination of peimine and peiminine in Fritillaria thunbergii by HPLC-ELSD

摘要: 目的利用HPLC-ELSD测定贝母类药材中的贝母素甲和贝母素乙。方法XTerra RP18色谱柱(150 mm×3.9 mm ID, 5 μm)，流动相为乙腈-10 mmol·L^-1 NH₄HCO₃(浓氨水调至pH 10.10)=A∶B系统，梯度洗脱，以ELSD检测。结果贝母素甲在1.09-4.36 μg，贝母素乙在1.04-4.16 μg与峰面积的对数值呈线性关系，回收率分别为98.96%(n=4)，RSD 1.01%；98.40%(n=4)，RSD 2.63%。结论本方法能够很好地测定浙贝母药材中的主要生物碱贝母素甲、贝母素乙，可用于浙贝母药材的质量控制。

Abstract: AimTo determine peimine and peiminine in Fritillaria drug simultaneously by RP-HPLC-ELSD. MethodsHPLC was carried out with a Waters Alliance, Model 2690,equipped with XTerra RP18 column (150 mm×3.9 mm ID, 5 μm) and evaporated light scattering detector. The mobile phase (acetonitrile-10 mmol·L^-1 NH₄HCO₃ adjusted to pH 10.10 by ammonia solution) was eluted in gradient mode. ResultsThe recoveries of peimine and peiminine were 98.96% (n=4), with RSD 1.01% and 98.40% (n=4), with RSD 2.63%, respectively. ConclusionThe method is simple, sensitive and reliable. It can be used for quantitative determination of Fritillaria drug.