沈祥春, 钱之玉, 陈琦, 王雅娟. 西红花酸对去甲肾上腺素所致原代培养心肌细胞能量代谢和凋亡的影响J. 药学学报, 2004, 39(10): 787-791.
引用本文: 沈祥春, 钱之玉, 陈琦, 王雅娟. 西红花酸对去甲肾上腺素所致原代培养心肌细胞能量代谢和凋亡的影响J. 药学学报, 2004, 39(10): 787-791.
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SHEN Xiang-chun, QIAN Zhi-yu, CHEN Qi, WANG Ya-juan. Protective effect of crocetin on primary culture of cardiac myocyte treated with noradrenaline in vitroJ. Acta Pharmaceutica Sinica, 2004, 39(10): 787-791.
Citation: SHEN Xiang-chun, QIAN Zhi-yu, CHEN Qi, WANG Ya-juan. Protective effect of crocetin on primary culture of cardiac myocyte treated with noradrenaline in vitroJ. Acta Pharmaceutica Sinica, 2004, 39(10): 787-791.
shu

西红花酸对去甲肾上腺素所致原代培养心肌细胞能量代谢和凋亡的影响

Protective effect of crocetin on primary culture of cardiac myocyte treated with noradrenaline in vitro

    摘要
  • 摘要: 目的研究西红花酸对去甲肾上腺素(NE)诱导原代培养心肌细胞能量代谢障碍和细胞凋亡的保护作用。方法1 μmol·L-1 NE损伤原代培养的心肌细胞,检测细胞培养上清液的LDH、心肌细胞ATPase、线粒体琥珀酸脱氢酶(MSDH)的活力,线粒体膜电位的变化,流式细胞仪检测细胞凋亡,观察西红花酸保护作用。结果模型组细胞培养上清液LDH增加,心肌细胞MSDH和ATPase的活力降低,线粒体膜电位降低,心肌细胞凋亡率增加。西红花酸明显降低培养上清液LDH增加,提高MSDH和ATPase的活力、线粒体膜电位的水平,对心肌细胞的凋亡具有明显的保护作用。结论西红花酸对NE所致的心肌细胞能量代谢障碍和凋亡具有明显的保护作用。

     

    Abstract: AimTo investigate the cardio-protective effect of crocetin on primary culture of cardiac myocyte treated with noradrenaline. MethodsAfter adding crocetin, the primary culture of cardiac myocyte was injured by 1.0 μmol·L-1 noradrenaline. The activity of lactic dehydrogenase (LDH), mitochondrion succinic dehydrogenase (MSDH) and ATPase were assayed. The mitochondrion membrane potential was detected by Rh123. The percentage of cardiac myocyte apoptosis was observed by flow cytometry. ResultsCrocetin significantly decreased the activity of LDH in culture supernatant, increased the activity of MSDH, ATPase (Na+-K+ATPase, Ca2+ATPase) and mitochondrion membrane potential. Conclusion Crocetin could alleviate the disturbance of energy metabolism and decrease the percentage of apoptosis of cardiac myocyte treated with noradrenaline.

     

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