This study is to investigate the effect of diet and gene on blood-fat trait of the individual mouse.  One hundred and twenty mice were fed with high fat emulsion for 4 weeks.  Then the genotypes of Glut-4 were   analyzed by PCR-SSCP to investigate the effect on blood-fat traits and some organ performance of mice.  After fed with high fat emulsion for 4 weeks, 98 mice suffered from hyperlipidemia, while 22 mice did not.  And a T/A mutation was found in 188 targeted fragments we amplified at 8 521 site, and association analysis showed that the mice carried BB genotype had higher TC, TG and LW/BW level than those of the mice with AA    genotype (P < 0.05).  Different Glut-4 genotypes show different sensitivities to high fat emulsion on mice.