岳旺, 张芳, 王蕾, 方选, 刘艳霞, 南胜. 一种新型呕吐动物模型水貂J. 药学学报, 2003, 38(2): 89-91.
引用本文: 岳旺, 张芳, 王蕾, 方选, 刘艳霞, 南胜. 一种新型呕吐动物模型水貂J. 药学学报, 2003, 38(2): 89-91.
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YUE Wang, ZHANG Fang, WANG Lei, FANG Xuan, LIU Yan-xia, MINAMI Masaru. A new vomiting animal modelminkJ. Acta Pharmaceutica Sinica, 2003, 38(2): 89-91.
Citation: YUE Wang, ZHANG Fang, WANG Lei, FANG Xuan, LIU Yan-xia, MINAMI Masaru. A new vomiting animal modelminkJ. Acta Pharmaceutica Sinica, 2003, 38(2): 89-91.
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一种新型呕吐动物模型水貂

A new vomiting animal modelmink

    摘要
  • 摘要: 目的 用水貂建立一种新型呕吐动物模型。方法 将水貂随机分组,给予3种经典致呕剂顺铂、阿朴吗啡、硫酸铜及X射线全身照射,测定致吐反应和抗呕吐药的抗呕吐反应,最后取水貂肠管进行5-HT免疫组化检测。结果顺铂(7.5 mg·kg-1,ip)、阿朴吗啡(1.6 mg·kg-1,sc)和硫酸铜(40 mg·kg-1,ig)3种致呕剂均使水貂发生恶心、干呕和呕吐,顺铂引起水貂呕吐的阈值剂量为5 mg·kg-1。昂丹司琼和甲氧氯普胺可抑制顺铂和阿朴吗啡所致的干呕、呕吐。5-HT免疫组化结果提示,肠道EC细胞5-HT释放参与呕吐的发生机制。结论水貂可作为一种新型呕吐模型,这对于抗呕吐机制和新药筛选研究有意义。

     

    Abstract: AimTo establish a new, reliable vomiting model in minks. MethodsAdult male minks (Mustela vison) were randomly divided into groups (n=6). Cisplatin, apomorphine, copper sulfate and X-radiation were used to establish vomiting model. Retching and vomiting were observed after the vomiting models were given anti-vomiting agents. After the behavioral experiment, assay of 5-HT in the ileum was performed by immunohistologic method. ResultsCisplatin 7.5 mg·kg-1 ip, apomorphine 1.6 mg·kg-1 sc and copper sulfate 40 mg·kg-1 ig were shown to evoke vomiting. Retching and vomiting were significantly inhibited in ondansetron and metoclopramide pretreated minks (P<0.05, P<0.01). ConclusionAs a new vomiting model, minks may be of great value in studying vomiting mechanism and screening new antiemetic drugs.

     

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