Abstract: Wifortrine and Euonine isolated from Tripterygium wilfordii Hook. f. Wilfortrine and euonine(40,80 mg/(kg, d)×4d ip) showed marked depressant effects oft humoral mediated immunity using the hemolysin reaction as indices. Wilfortrine (160 mg/kg.d×9d ip) exhibited a depressant effect on graft vs host reaction (GVHR) , but wilfortrine(80 mg/(kg.d)×9d ip) showed no definite effect, euonine(80 mg/(kg.d)×10d ip) showed marked suppressant effects on DNCB induced delayed hypersensitivity reaction on skin in mice. Wilfortrine and Euonine (80 mg/(kg.d)×4d ip)significantly decreased the clearnce rate of charcoal particles and the weights of spleen and thymus.