Abstract: AimTo evaluate the effects of dipfluzine on the expressions of E-selectin, P-selectin, and ICAM-1 and the infiltration of polymorphonuclear leukocytes in brain ischemia-reperfusion rats. MethodsThe model of focal cerebral ischemia was established with the Zea-Longa occluding suture. Dipfluzine (0.25, 0.5 and 1 mg·kg^-1), flunarizine 0.5 mg·kg^-1 and solvent were injected separately into lingual vein at 30 min after ischemia. The occluding suture was slowly taken away to cause reperfusion at 1 h after ischemia. Rats were decapitated under anesthesia at 24 h after ischemia-reperfusion and brains were immediately removed to do the following procedures. Effects of dipfluzine on morphology of the brain tissue were observed through hematoxylin-eosin (HE) staining. By immunohistochemistry and flow cytometry technique and biochemical method, effects of dipfluzine on P-selectin, E-selectin, ICAM-1 and myeloperoxidase (MPO) were observed. ResultsDipfluzine could relieve pathological damages in the brain tissue after ischemia-reperfusion, and reduce the expressions of E-selectin, P-selectin and ICAM-1 and activities of MPO in dose-dependent manner. ConclusionDipfluzine depresses the expressions of P-selectin, E-selectin，and ICAM-1, which are correlated with their effects on the activities of MPO, suggesting that dipfluzine has anti-inflammation effect in certain extent and could protect brain tissue from ischemia-reperfusion injury.