雷公藤倍半萜生物碱的研究

STUDY OF SESQUITERPENE ALKALOIDS FROM TRIPTERYGIUM WILFORDII HOOK.F.

摘要: 目的　研究雷公藤(Tripterygium wilfordii Hook.f.)的化学成分。方法　应用各种色谱技术进行分离纯化，用UV，IR，MS，HRMS，¹HNMR，¹³CNMR(COM和OFR)，¹H-¹HCOSY，¹H-¹³CCOSY，2D-NOESY，¹H-¹³CCOLOC鉴定化合物。并进行初步的药理实验。结果　分离并鉴定了2个生物碱：euonine(I)和雷公藤康碱(IIwilfordconine)。结论　II为一种新的倍半萜生物碱，药理实验证明该生物碱有免疫抑制活性，并对白血病细胞有抑制作用。

Abstract: AIM　To study the chemical composition of Tripterygium wilfordii Hook.f. METHODS Column chromatography was used to separate the chemical constituents. UV, IR, MS, HRMS, ¹HNMR, ¹³CNMR (COM and OFR), ¹H-¹H COSY, ¹H-¹³C COSY, 2D-NOESY and ¹H-¹³C COLOC were used to determine the structures of the isolated constituents. RESULTS　Two sesquiterpene alkaloids were isolated and their structures were elucidated as euonine and wilfordconine on the basis of spectral evidence. CONCLUSION Wilfordconine, a new sesquiterpene alkaloid,was shown to be immunosuppressive.