Abstract: A new analytical method for the separation and determination of seven aqueous depsides in salria milliorrhiza by HPTLC has been developed. The seven depsides are protocatechualdehyde, caffeic acid, methyl rosmarinate, rosmarinic acid, salvianolic acid A, B and C. Using chloroform—ethyl acetate—benzene—formic acid (2.4:2:1:0.6) as developing solvent A. protocate-chualdehyde was separated; using chloroform—ethyl acetate—benzene—formic acid—methanol (1.5:2:1:1:0.1) as developing solvent B, the other six constituents were well separated. The aqueous depsides were detected at the wave lengths of λ_s=300 nm and λ_R=240. This method is simple. rapid, sensitive and accurate. The contents of seven depsides in several Salria species were determined.