张胜华, 陈静, 江敏, 甄永苏. 力达霉素诱导人胃癌BGC823细胞凋亡和抑制裸鼠移植瘤生长J. 药学学报, 2008, 43(6): 601-604.
引用本文: 张胜华, 陈静, 江敏, 甄永苏. 力达霉素诱导人胃癌BGC823细胞凋亡和抑制裸鼠移植瘤生长J. 药学学报, 2008, 43(6): 601-604.
shu
ZHANG Sheng-hua, CHEN Jing, JIANG Ming, ZHEN Yong-su. Lidamycin induces apoptosis of human gastric carcinoma BGC823 cells and inhibits xenograft growth in nude miceJ. Acta Pharmaceutica Sinica, 2008, 43(6): 601-604.
Citation: ZHANG Sheng-hua, CHEN Jing, JIANG Ming, ZHEN Yong-su. Lidamycin induces apoptosis of human gastric carcinoma BGC823 cells and inhibits xenograft growth in nude miceJ. Acta Pharmaceutica Sinica, 2008, 43(6): 601-604.
shu

力达霉素诱导人胃癌BGC823细胞凋亡和抑制裸鼠移植瘤生长

Lidamycin induces apoptosis of human gastric carcinoma BGC823 cells and inhibits xenograft growth in nude mice

    摘要
  • 摘要: 观察力达霉素(LDM)对人胃癌BGC823细胞的诱导凋亡作用及体内抗肿瘤活性。采用MTT法观察LDM对人胃癌BGC823细胞增殖的抑制作用。利用Annexin V-FITC/PI双染结合流式细胞仪和脱氧核糖核酸末端转移酶介导的缺口末端标记技术检测细胞凋亡的改变。采用Western blotting法检测细胞中VEGF蛋白的表达情况。建立裸鼠胃癌皮下移植瘤模型，观察LDM的体内抗肿瘤活性。LDM能够明显抑制BGC823细胞增殖，诱导细胞凋亡，降低细胞VEGF蛋白的表达，抑制胃癌裸鼠移植瘤的生长。LDM剂量0.02和0.04 mg·kg-1的抑瘤率分别为57%和72%(P<0.01)。LDM可诱导胃癌细胞凋亡并抑制裸鼠移植肿瘤的生长。

     

    Abstract: To investigate the effect of lidamycin (LDM) on human gastric carcinoma BGC823 cells and xenograft growth in nude mice, MTT (methyl thiazolyl tetrazolium) assay was used to determine the inhibition of BGC823 cell proliferation by LDM. Induction of apoptosis was studied by flow cytometry and TUNEL assay. The expression of VEGF was detected by Western blotting analysis. Athymic nude mice were used to determine in vivo antitumor activity. Proliferation inhibition and apoptosis induction were studied in lidamycin-treated cells. The expression of VEGF in BGC823 cells decreased in a dose-dependent manner. LDM at 0.02 mg·kg-1 and 0.04 mg·kg-1 suppressed the growth of BGC823 xenografts in nude mice by 57% and 72%, respectively. LDM potently induces apoptosis in human gastric carcinoma BGC823 cells and inhibits xenograft growth.

     

    • HTML全文
    • 参考文献(0)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回