雷藤氯内酯醇对正常人及类风湿关节炎患者周围血单个核细胞及滑膜细胞产生免疫球蛋白的影响

EFFECTS OF TRIPCHLOROLIDE (T₄) OF TRIPTERYGIUM WILFORDII HOOK ON THE PRODUCTION OF IMMUNOGLOBULINS BY PERIPHERAL BLOOD MONONUCLEAR CELLS AND BY SYNOVIAL CELLS OF RHEUMATOID ARTHRITIS PATIENTS IN VITRO

摘要: 研究了10例正常人和6例类风湿关节炎(RA)患者外周血单个核细胞(PBMC)及3例RA患者消化的单个滑膜细胞(DSSC),经雷公藤的有效单体雷藤氯内酯醇(T₄)处理2h对其分泌免疫球蛋白(Ig)的影响。结果显示:T₄在浓度为5～35ng·mL^-1对正常人PBMC分泌Ig有明显抑制作用,呈剂量依赖趋势。在25ng·mL^-1浓度下,T₄对RA患者PBMC及DSSC分泌Ig均有明显抑制作用。

Abstract: Tripchlorolide(T₄) is an active ingredient recently isolated from Tripterygium Wilfordii Hook. The in vitro effects of T₄ on the peripheral blood mononuclear cells(PBMC) of healthy persons(n=10), the digested single synovium cells(DSSC)(n=3) and PBMC(n=6) from RA patients on production of immunoglobulins(Ig) were studied using Elisa method. The results showed that T₄ at concentrations from 5 ng·ml^-1 to 35 ng·ml^-1 significantly reduced the production of Ig by PWM-stimulated PBMC of healthy persons in a dose dependent manner. At concentration of 25 ng·ml^-1, T₄ also reduced the Ig secretion of RA-PBMC and DSSC. It is hoped that T₄ would be an encouraging new drug of herbal nature for the treatment of RA.