胶束电动毛细管色谱法分离和测定几种大黄含量

MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY SEPARATION AND DETERMINATION OF CHINESE TRADITIONAL MEDICINE──SEVERAL RHEUM SPECIES

摘要: 建立了胶束电动毛细管色谱法分离和测定几种大黄中大黄素、芦荟大黄素、大黄酸含量的测定方法。缓冲液为含0.025mol·L^-1十二烷基磺酸钠(简称SDS)的0.025mol,L-13-环己氨基-1-丙烷磺酸(简称CAPS)-乙腈(100:10),pH0.96。线性范围:芦荟大黄素为0.0025～0.0175mg·ml^-1(r=0.9999),大黄素为0.005～0.05mg·ml^-1(r=0.9999),大黄酸为0.005～0.035mg·ml^-1(r=0.9996)。回收率:芦荟大黄素为98.2%～101.8%,大黄素99.9%～101.9%,大黄酸99.2/～101.2%。此法简便、快速、重现性好。

Abstract: A micellar electrokinetic capillary chromatographic(MECC) method for the separation and determination of emodin, aloe-emodin and rhein in several Rheum species was estab-lished. The buffer solution used in this method was a mixture of 0.025 mol·L^-1 3-cyclohexylamino-1-propane-sulfonic acid(containing 0.025 mol·L^-1 sodium dodecyl sulfate)-acetonitrile(100:10 ) at pH 10. 9 The linear calibration range was 2.5～17.5μg,ml^-1(r=0.9999) for aloe-emodin,0～50μg· ml^-1(r=0.9999) for emodin and 5.0～35μg·ml^-1(r=0.9996)for rhein. The recovery was 98.2%～101.8%for aloe-emodin, 99.9%～101.9%for emodin and 99.2%～101,2%for rhein. This method is simple, rapid and reproducible.