Abstract: A Modified iodometric method for determination of Tibione,was found to be better than alkalimetric method or argentimetric method in its rapidity,simplicity and reproducibility. The method suggested is as follows: Weigh accurately about 0.1 g of tibione into a 50 ml volumitric flask,add 20 ml 1 N sodium hydroxide,mix well,and dilute to the mark with water.Transfer 10 ml of the solution (equivalent to 0.02 g of Tibione)into a glass-stopped flask,add 10 ml 1 N sodium hydroxide and 25 ml 0.1N iodine solution rapidly,and allow to stand in the dark for 10 minutes at 2—4c. Then acidify with 10 ml 1N hydrochloric acid and titrate the liberated iodine with sodium thiosulfate solution,using starch solution as indicator.One 0.1N iodine solution is equivalent to 0.002954 g Tibione.