Abstract: AIMTo study the chemical constituents of the rhizomes of Coeloglossum viride (L.) Hartm. var. bracteatum (Willd.) Richter. METHODSThe compounds were mainly isolated with normal phase and reverse phase column chromatographic methods and HPLC. Their structures were elucidated on the basis of physical constants and spectral analysis (UV, IR, EI-MS, positive and negative FAB-MS, APCI-MS, ¹HNMR, ¹³CNMR, DEPT, ¹H-¹H COSY, HMQC and HMBC). RESULTSEight compounds were obtained from the ethanolic extract of the rhizomes of this plant, dactylorhin B (I), loroglossin (II), dactylorhin A (III), militarine (IV), coelovirin A (V), gastrodin (VI), thymidine (VII) and quercetin-3,7-di-O-β-D-glucopyranoside (VIII). CONCLUSIONAll the compounds were obtained from this plant and genus Coeloglossum for the first time. V named coelovirin A, is a new compound.