Abstract: Nineteen anabolic steroids were separately administerd to healthy men. Anabolic steroid metabolites were isolated and extracted from the collected urine samples following an integrated procedure. The main metabolic pathways of these drugs were made clear after the investigation by GC-MS. Based on the obtained chromatographic- mass spectrometric data of anabolic steroids, a method for large scale and routine analysis of anabolic steroids was set up.