徐峰, 张洪泉. 白细胞介素-1受体拮抗剂对卵白蛋白致敏的哮喘豚鼠嗜酸性粒细胞凋亡的影响J. 药学学报, 2003, 38(9): 661-664.
引用本文: 徐峰, 张洪泉. 白细胞介素-1受体拮抗剂对卵白蛋白致敏的哮喘豚鼠嗜酸性粒细胞凋亡的影响J. 药学学报, 2003, 38(9): 661-664.
shu
XU Feng, ZHANG Hong-quan. Effects of interleukin-1 receptor antagonist on the apoptosis of eosinophil in guinea pig with asthmaJ. Acta Pharmaceutica Sinica, 2003, 38(9): 661-664.
Citation: XU Feng, ZHANG Hong-quan. Effects of interleukin-1 receptor antagonist on the apoptosis of eosinophil in guinea pig with asthmaJ. Acta Pharmaceutica Sinica, 2003, 38(9): 661-664.
shu

白细胞介素-1受体拮抗剂对卵白蛋白致敏的哮喘豚鼠嗜酸性粒细胞凋亡的影响

Effects of interleukin-1 receptor antagonist on the apoptosis of eosinophil in guinea pig with asthma

    摘要
  • 摘要: 目的研究白细胞介素-1受体拮抗剂(IL-1ra)，对慢性哮喘豚鼠肺内嗜酸性粒细胞凋亡及相关机制的影响。方法建立致敏豚鼠模型，连续吸入不同浓度IL-1ra,用卵白蛋白引喘豚鼠，连续8 d，分离血液、肺泡灌洗液(BALF)中嗜酸性粒细胞，计数嗜酸性粒细胞，用放射免疫法测定上清液中阳离子蛋白（ECP），酶连免疫法（ELISA）测血清中IL-5，荧光显微镜观察嗜酸性细胞凋亡。结果IL-1ra能明显减少肺部嗜酸性粒细胞浸润，降低血清、肺泡灌洗液中ECP水平及血清中IL-5的含量，促进嗜酸性细胞凋亡。结论雾化吸入IL-1ra可以通过改变嗜酸性粒细胞的活性及浸润程度，达到治疗哮喘的目的。

     

    Abstract: AimTo evaluate the effect of interleukin-1 receptor antagonist(IL-1ra) on apoptosis and associated mechanism of eosinophil in guinea pig with asthma. MethodsA model of guinea pig with asthma was established. After inhalation of different concentrations of IL-1ra, asthma was induced in the guinea pig for 8 days, the concentration of eosinophil cationic protein (ECP) in serum and bronchoalveolar lavage fluid (BALF),IL-5 in serum ,the eosinophil counts and apoptosis were assayed by radioimmunology, enzyme-linked immunosorbent assay(ELISA), fluoromicroscope and light microscope. ResultsIL-1ra indirectly decreased the level of IL-5 in serum, improved the apoptosis of eosinophil(EOS) in lung, then decreased the level of ECP in serum and BALF. ConclusionInhalation of nebulized IL-1ra showed protective effect against asthma through change of the activity and infiltration of EOS in lung.

     

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