西陵知母中甾体皂苷的分离与鉴定

ISOLATION AND IDENTIFICATION OF STEROIDAL SAPONINS FROM ANEMARRHENA ASPHODELOIDES BUNGE.

摘要: 目的:从新鲜西陵知母(Anemarrhena asphodeloides Bunge.)根茎中分离出有生理活性的化学成分—甾体皂苷。方法:用柱色谱法分离纯化,通过理化方法及光谱分析(UV,IR,¹HNMR,¹³CNMR,EI-MS,ESI-MS,HMBC,HMQC)鉴定化学结构。结果:从新鲜西陵知母根茎的乙醇提取物中分离得到西陵知母皂苷A与B,其化学结构分别为萨尔萨皂苷元-3-O-β-D-葡糖基(1→2)-O-β-D-半乳糖苷和萨尔萨皂苷元-3-O-β-D-葡糖基(1→3)-O-β-D-葡糖基(1→2)-O-β-D-半乳糖苷。结论:西陵皂苷B为一新化合物。

Abstract: AIM: To isolate and elucidate the chemical structures of he steroidal saponins possessing physiological activity from the fresh rhizoma of Anemarrhena asphodeloides Bunge. (Xilingzhimu). METHODS: The constituents were isolated and purified by repeated column chromatography on silica gel. The molecular structures were elucidated on the basis of chemical evidences and spectral analysis (UV, IR, EI-MS, EIS-MS, ¹HNMR,¹³CNMR, HMBC and HMQC). RESULTS: Two steroidal saponins, xilingsaponin A and B were obtained from the fresh rhizoma of Xilingzhimu. They were elucidated as sarsasapogenin-3-O-β-Dglucopyranosyl-(1→3)-β-D-glucopyranosyl-(1→2)-β-D-galactopyranoside respectively. CONCLUSION: Xilingsaponin B is a new compound.