This paper is to report the investigation on the metabolic behavior of Staphylococcus aureus (S. aureus) after given Qingkailing injection, and with the aim of seeking for a new quality control method based on biological assessment.  The growth thermogenic curves of S.aureus were determined by microcalorimetry and analyzed by computer.  The results showed that in the concentration range of (0 − 5.00%), the growth  thermogenic curves of S. aureus were declined and removed back with increasing dosage of Qingkailing injection; the main parameters (T₁, T₂, k₁, P₁, P₂ and I ) and the dosage of Qingkailing injection have good correlation.  The 50% inhibiting dosage is 3.26 %, and the optimal inhibiting dosage is 5.47%.  Difference could be detected among the Qingkailing injection samples from different factories and different batches.  It is proved that Qingkailing injection could inhibit the metabolic behavior of S.aureus, and microcalorimetry might be applied in the quality assessment of Qingkailing injection.