两种头孢菌素对大肠杆菌生长代谢作用的微量热研究

Microcalorimetric investigation of two cephalosporins on colon bacteria activity

摘要:
用微量量热法测定了37 ℃时头孢拉定和头孢他定对大肠杆菌DH5α代谢作用的产热曲线。根据产热曲线, 分别得到了大肠杆菌DH5α在不同浓度的头孢拉定和头孢他定作用下的生长速率常数k、抑制率I、最大产热功率P_m以及最大产热功率所对应的时间t_m等热动力学参数, 从热动力学的角度探讨了头孢拉定和头孢他定对大肠杆菌DH5α代谢作用的影响。实验证明, 头孢他定对大肠杆菌的抑制作用比头孢拉定更强。

Abstract:
The effects of cephradinum and ceftazidime on the metabolism of Escherichia coli (E. coli) DH5α was determined by microcalorimetry. The microbial activity was recorded as power-time curves through an ampoule method with a TAM Air Isothermal Microcalorimeter at 37 ℃. The parameters such as the growth rate constant (k), inhibitory ratio (I), the maximum power output (P_m) and the time (t_m) corresponding to the maximum power output were calculated. The results show that the ceftazidime has a better inhibitory effect on E.coli DH5α than cephradinum.