颠茄类生物碱的毛细管气相色谱和色—质联用分析

STUDIES ON ANALYSIS OF BELLADONNA ALKALOIDS BY CAPILLARY GC AND GC-MSD

摘要: 本文用毛细管气相色谱和气相色谱-质量选择检测器分析颠茄生物碱类(莨菪碱、东莨菪碱、山莨菪碱和樟柳碱)的研究。对颠茄生物碱与不同硅烷化试剂、反应能力、反应时间以及反应温度作了探索。结果表明,MSTFA是最好的硅烷化试剂。文内给出了这些生物碱的原型和硅烷化衍生物的保留值和它们的特征性碎片离子。这些数据有助于该类生物碱的鉴定。方法已初步应用于这些生物碱在生物合成中的转化研究。此外,还讨论了颠茄生物碱类TMS衍生化的优点。

Abstract: A method for analyzing belladonna alkaloids——hyoscyamine,scopolamine, anisodamine and anisodine, by means of capillany GC and GC-MSD wasdescribed. The retention data and characteristic ions of the parent and TMS derivatives ofthese alkaloids are given and will be very useful for identification of unknown alkaloids.The derivatization of different silylation reagents was compared, and the derivatizationwith MSTFA was found to be better than with BSA and BSTFA. The method has beenused for studying biotransformation of these alkaloids in biosynthesis. Analysis ofanisodamine and anisodine by CGC and GC-MS has not been found in the literature Inaddition, the advantages of TMS derivatization are also discussed.