左旋黄皮酰胺对冈田酸和β淀粉样肽25-35神经毒性的保护作用
Protective effect of (-)clausenamide against neurotoxicity induced by okadaic acid and β-amyloid peptide25-35
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摘要: 探讨左旋黄皮酰胺对冈田酸(okadaic acid，OA)诱导的人神经瘤细胞(SH-SY5Y)和去卵巢(ovariectomy，OVX)及单侧侧脑室注射Aβ25-35所致神经元损伤的保护作用。通过MTT试验、LDH释放测定试验、Hoechst 33258荧光染色试验以及SH-SY5Y细胞检测，考察左旋黄皮酰胺拮抗冈田酸诱导的细胞毒作用。通过避暗试验、电镜检测、Nissl体染色及HE染色，考察左旋黄皮酰胺对去卵巢及侧脑室注射Aβ25-35大鼠神经元的保护作用。左旋黄皮酰胺可明显拮抗冈田酸诱导的细胞毒作用，提高去卵巢及侧脑室注射Aβ25-35大鼠的学习记忆能力，保护海马及皮层神经元。左旋黄皮酰胺可拮抗冈田酸及Aβ25-35诱导的神经毒性，具有神经保护作用。Abstract: This study is to investigate the protective effect of (-)clausenamide against the neurotoxicity of okadaic acid in SH-SY5Y cell line, and injection β-amyloid peptide25-35 (Aβ25-35) to the cerebral ventricle in ovariectomy (OVX) rats. MTT assay, LDH assay, and Hoechst 33258 staining were used to detect the effect of (-)clausenamide on the toxicity of okadaic acid in SH-SY5Y cell line. The animal model was induced by ovariectomized and injection of Aβ25-35 in the cerebroventricle of rats. The effect of (-)clausenamide on learning and memory deficiency was observed by step-through test. Electron microscope, Nissl body staining, and HE staining were used to examine the morphological changes in hippocampus and cerebral cortex neurons. Pretreatment of (-)clausenamide and LiCl decreased the rate of cell death from MTT, LDH release, and apoptosis from Hoechst 33258 staining in SH-SY5Y cell line. The step-through tests showed (-)clausenamide could improve the ability of learning and memory. The Nissl body staining and HE staining experiments also showed the neuroprotective effects of (-)clausenamide on the neurons of hippocampus and cerebral cortex. (-)Clausenamide has the protective effects against the neurotoxicity induced by okadaic acid and Aβ25-35.
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