肾上腺素在碳糊电极上的吸附伏安测定法研究

STUDY OF ADSORPTIVE VOLTAMMETRY FOR ADRENALINE ON CARBON PASTE ELECTRODE

摘要: 为研究肾上腺素的电化学行为及其检测方法,在0.5mol·L^-1H₂SO₄底液中,用碳糊电极吸附伏安法测定肾上腺素,阳极峰电位为0.58V(vs.SCE),峰电位与肾上腺素的浓度在5.0×10^-9～1.0×10^-4mol·L^-1范围内呈良好的线性关系。此法检测下限为2.5×10^-9mol·L^-1,回收率为93.75%～103.33%,相对标准偏差为3.1%(n=12)。用本法对盐酸肾上腺素注射液进行了测定,获得满意结果。本文对反应机理进行了初步探讨,肾上腺素在碳糊电极上是一个两电子、两质子的不可逆过程。

Abstract: Adrenaline is determined by adsorptive voltammetry using a carbon paste electrode in a base solution of 0.5 mol·L^-1 H₂SO₄. The peak potential of anode is 0.58 V (vs.SCE). There is a good linear relationship between the peak current and the concentration of adrenaline in the range of 5.0×10^-9～1.0×10^-4 mol·L^-1. The detection limit of the method is 2.5×10^-9 mol·L^-1. The recovery is 93.75%～103.33%, and the relative standard deviation is 3.1% (n=12). The adrenaline hydrochloride injection has been determined with good results by this method. The possible reaction mechanism is discussed. The electrode reaction of adrenaline is irreversible process with two electrons and two protons on the carbon paste electrode.