知母的皂苷成分

SAPONINS FROM THE RHIZOMES OF ANEMARRHENA ASPHODELOIDES BGE.

摘要: 目的：研究知母的化学成分，寻找新的活性物质。方法：利用大孔树脂柱色谱、硅胶柱色谱及高效液相色谱等手段进行分离，根据化合物的理化性质及光谱(IR，FAB-MS，ESI-MS，¹HNMR，¹³CNMR，DEPT，HMQC，HMBC，NOESY和ROESY)数据鉴定结构。结果：从中药知母分离得到两种甾体皂苷，确定其结构是：(5β，25S)-螺甾烷-3β，15α，23α-三醇-3-O-β-D-吡喃葡萄糖基(1→2)-β-D-吡喃半乳糖苷(1)和(5β，25S)-螺甾烷-3β，23α-二醇-3-O-β-D-吡喃葡萄糖基(1→2)-β-D-吡喃半乳糖苷(2)。结论：1和2均是新化合物，分别命名为知母皂苷F和知母皂苷G。

Abstract: AIM: To investigate the constituents of the rhizomes of Anemarrhena asphodeloides Bge. and look for new active compounds. METHODS: Compounds were isolated with porous resin, silica gel, HPLC and so on, and identified by chemical and spectroscopic evidence. RESULTS: Two steroidal saponins were obtained, and their structures were shown to be (5β,25S)-spirostan-3β,15α,23α-triol-3-O-β-D-glucopyranosyl(1→2)-β-D-galactopyranoside (1) and (5β,25S)-spirostan-3β,23α-diol-3-O-β-D-glucopyranosyl(1→2)-β-D-galactopyranoside (2). CONCLUSION: They were new compounds, named as timosaponin-F (1) and timosaponin-G (2), respectively.