吲哚美辛和美洛昔康对脂多糖诱导的小鼠腹腔巨噬细胞NF-κB的抑制作用

INHIBITORY EFFECTS OF INDOMETHACIN AND MELOXICAM ON NF-κB IN MOUSE PERITONEAL MACROPHAGES

摘要: 目的　研究非甾体抗炎药吲哚美辛(indomethacin)和美洛昔康(meloxicam)对细菌脂多糖(LPS)诱导表达的C57小鼠腹腔巨噬细胞核转录因子NF-κappaB(NF-κB)的抑制作用。方法　NF-κB的测定采用电泳迁移率改变法。结果　小鼠腹腔巨噬细胞经LPS诱导后细胞NF-κB含量明显增高。Indomethacin和meloxicam在10^-5-10^-7mol.L^-1浓度下可明显降低LPS激活的小鼠腹腔巨噬细胞NF-κB活化。结论　Indomethacin和meloxicam对NF-κB活化的抑制作用可能是两者的抗炎作用机理之一。

Abstract: AIM　To study the inhibitory effects of indomethacin and meloxicam on NF-κB from lipopolysaccharide (LPS) stimulated peritoneal macrophages of mice. METHODS　NF-κB was measured with the method of electrophoretic mobility shift assay (EMSA). RESULTS　After induction by LPS at the concentrations of 1 and 3 μg.mL^-1, the NF-κB content of the mouse peritoneal macrophages increased markedly. Indomethacin and meloxicam, at the concentrations of 10^-7-10^-5 mol.L^-1, decreased the activation of NF-κB at the concentrations of 1 and 3 μg.mL^-1 in activated mouse peritoneal macrophages induced with LPS at the concentrations of 1 and 3 μg.mL^-1. CONCLUSION　The inhibitory effects of indomethacin and meloxicam on NF-κB activation may be one of their mechanisms of antiinflammatory actions.