灵芝孢子粉多糖Lzps-1的化学研究及其总多糖的抗肿瘤活性

Chemistry of polysaccharide Lzps-1 from Ganoderma lucidum spore and anti-tumor activity of its total polysaccharides

摘要: 目的研究微波软化灵芝孢子粉中的多糖组分及其抗肿瘤活性。方法用水提取微波软化灵芝孢子粉总多糖，总多糖经分级沉淀得到多糖组分Lzps-C，再采用DEAE-cellulose和Sephadex G-50柱色谱进行分离纯化，用化学和光谱方法分析其结构。结果从微波软化灵芝孢子粉的水提物中分得一个多糖Lzps-1，其平均分子量为8 000，为葡聚糖。微波软化灵芝孢子粉的水提物得到的总多糖Lzps对小鼠Lewis肺癌、小鼠S₁₈₀肉瘤有较好的抑制作用，并能明显提高荷Lewis肺癌小鼠NK活性。结论Lzps-1是首次从灵芝孢子粉中分离得到。Lzps具有抗癌活性。

Abstract: AimTo study the structure and anti-tumor activity of polysaccharide from Ganoderma lucidum spore treated with microwave. MethodsDEAE-cellulose and Sephadex G-50 column chromatography were used to isolate and purify the polysaccharide whose structure was characterized by using chemical and spectral methods. Results and ConclusionOne polysaccharide, named Lzps-1 was obtained from the water extract, with its molecular weight estimated by HPGPC to be 8 000. Its structure was investigated to be glucan. The total polysaccharides, Lzps processed antitumor activity against sarcoma 180 and Lewis lung cancer in mice and enhanced the NK cell activity. Lzps-1 is obtained for the first time from Ganoderma spore Lzps has anti-tumor activity.