Abstract: Two new sesquiterpenoids, named as tyromols A and B(1 and 2), were isolated from cultures of basidiomycete Tyromyces chioneus, along with two previously reported 15-hydroxy-6α, 12-epoxy-7β, 10αH, 11βH-spiroax-4-ene(3)and agripilol C(4). Compounds 1-4 were separated and purified by silica gel, RP-18, Sephadex LH-20 column chromatography. Their structures were elucidated on the basis of extensive spectroscopic analysis including IR, MS, 1D and 2D NMR experiments.