间硝苯地平对血管紧张素Ⅱ促进血管平滑肌细胞增殖和蛋白质合成的影响

INHIBITORY EFFECTS OF M-NIFEDIPINE ON ANGIOTENSIN ⅡSTIMULATED PROLIFERATION AND PROTEIN SYNTHESIS IN CULTURED RABBIT AORTIC SMOOTH MUSCLE CELLS

摘要: 以培养血管平滑肌细胞(vascularsmcothmusclecell,VSMC)为模型,观察了间硝苯地平(m-nifedipine,m-Nif)对血管紧张素Ⅱ(angiotensinⅡ,ANGⅡ)促进VSMC增殖和蛋白质合成的影响。结果表明,m-Nif抑制ANGⅡ(100nmol·L^-1)引起VSMC³Hthymidine和³Hleucine参入,并呈剂量依赖性。m-Nif(2×10^-6mol·L^-1)可抑制ANGⅡ对VSMC的刺激、DNA及蛋白质合成速率,分别降低了46%,58%,53%。提示m-Nif可抑制ANGⅡ对VSMC增殖和蛋白合成的促进作用。

Abstract: By using rabbit aortic smooth muscle cells(VSMC)in culture as a model,theeffects of m-nifedipine(m-Nif)on the growth of VSMC were observed.The results showed that m-Nif inhibited ³H-thymidine and³H-leucine incorporation into DNA and protein,ecreased cellnumber in a concentration-dependent manner in angiotensinⅡ(ANGⅡ)induced VSMC.At theconcentration of 2 μmol·L^-1,m-Nif was found to reduce the ANGⅡ(100 nmol·L^-1)stimulated³H-thymidine,³H-leucine incorporation and cell number by 46%,58% and 53%,respectively。Our results suggest that m-Nif can inhibit ANG Ⅱ stimulated proliferation and protein synthesis incultured rabbit aortic VSMC.