用自旋捕捉技术检测争光霉素A6产生的活泼自由基
DETECTION OF SUPEROXIDE AND HYDROXY RADICALS FORMED IN BLEOMYCIN A6-Fe2+ SYSTEM BY SPIN TRAPPING
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摘要: 本文用自旋捕捉技术与ESR相结合的方法,研究了争光霉素A6-Fe2+复合物产生的活泼自由基。结果发现用NOS自旋捕捉剂可检测到该体系所产生的超氧阴离子自由基。在水溶剂中用PBN自旋捕捉到了羟基自由基。根据PBN-OH自旋加合物在水溶剂和甲醇溶剂中的超精细分裂常数,进一步确证了羟基自由基的生成。Abstract: The active radicals produced in Bleomycin A6-Fe2+ system have been studied by combination of spin trapping technique with ESR spectroscopy. It was found that the superoxide radicals could be detected by NOS in the above system. The hydroxyl radicals could be trapped by PBN in aqueous solution. In accordance with the parameters of ESR spectra of spin adducts of PBN-OH in aqueous and methyl alcohol solutions, the production of OH radical have been further demonstrated.
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