Abstract:
Rhei Rhizoma is a Chinese medicine with multiple botanical origins. There is a problem to identify it with conventional methods. To compare the characteristics of chloroplast
mat K gene sequences of different
Rheum species and authenticate inspected species, the
mat K gene sequences of different species from different origins were amplified, cloned, and sequenced. Genomic DNA of
Rheum plants was extracted using modified DNA extracted Kit and
mat K gene sequences were analyzed by ContingExpress, DNAman and MEGA5.0. The length of
mat K gene sequences of
Rheum palmatum,
R. tanguticum and
R. officinale were 1 518 bp containing 57 variable loci. According to the mutation sites,
R. palmatum,
R. tanguticum and
R. officinale were divided into different genotypes separately. Based on the established method according to the loci 587, 707, 838, we successfully identified the genuine
Rheum species from its adulterants.
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