Stealth tanshinone IIA-loaded solid lipid nanoparticles (TA-SSLNs) have been prepared and the influence of poloxamer 188 coating on in vitro phagocytosis and in vivo pharmacokinetics in rats were evaluated.  TA-SSLNs have been prepared by a nanoprecipitation/solvent diffusion method.  Poloxamer 188 was used as a stealth agent.  The physicochemical parameters of TA-SSLNs were characterized in terms of particle size, zeta potential, transmission electron microscopy and stability.  In vitro, phagocytosis was investigated by incubating TA-SSLNs and non-stealth tanshinone IIA-loaded solid lipid nanoparticles (TA-NSLNs) with murine macrophages.  In vivo, pharmacokinetics of TA-SSLNs and TA-NSLNs after a single dose intravenous injection to rat has   been studied.  The control was tanshinone IIA solution (TA-SOL).  The results showed that TA-SSLNs    had an average diameter of (91.3 ± 3.4) nm, zeta potential of (−19.7 ± 1.6) mV, drug loading of (4.7 ± 0.5) % and entrapment efficiency of (92.5 ± 2.1) %.  Phagocytosis studies showed significant differences between TA- SSLNs and TA-NSLNs and demonstrated that the poloxamer 188 coating could decrease the macrophage uptake.  In vivo experiments showed that the plasma concentration data of TA-SSLNs, TA-NSLNs and TA-SOL were all fitted to a two-compartment model.  Areas under curve (AUCs) of TA-NSLNs and TA-SSLNs were 1.28 and 3.70 times than that of TA-SOL, respectively.  TA-SSLNs had generated a long circulating time in blood with a mean residence time (MRT) of 5.286 h, compared to 3.051 h of TA-NSLNs and 0.820 h of TA-SOL.  Poloxamer 188 modification on solid lipid nanoparticles (SLNs) reduced opsonization by serum proteins and the macrophage uptake.  AUC of tanshinone IIA increased as a function of SLNs.  In addition, TA-SSLNs exhibited much longer circulation lifetimes for tanshinone IIA than TA-NSLNs.  The pharmacokinetic behavior of the incorporated drug can be modified by changing the surface characteristics of SLNs with the use of poloxamer 188.