Abstract: The optimal combination of paper chromatographic solvent systems of 22 kinds of amino acids was made successfully with the stepwise combination method on TRS-80 microcomputer. Three groups of optimal combination of solvent systems were selected. The numbers of optimal combination system are 7, 4, 2, 1; 17, 9, 4, 2; 21, 17, 4, 2. The 22 kinds of amino acids tested could be separated at a level that the difference of Rf × 100 values between any two samples is equal to 10 or more using each combination of the groups mentioned above. At the same time, numerical taxonomic technique-information content method was also used to select the optimal combination of the systems, but no satisfactory results were obtained.