促性腺激素释放多肽(GRP)对体外培养小鼠垂体分泌LH的影响

EFFECT OF GONADOTROPIN-RELEASING PEPTIDE (GRP) ON LH SECRETION OF MOUSE PITUITARY IN VITRO

摘要: 合成的促性腺激素释放多肽(GRP)及其类似物GRp～NH₂,Glu^7.9.14Lys^6.10GRP(6～14),phe¹⁴GRP(5～14)和phe¹⁴GRP浓度在0.05mmol·L^-1时,具有刺激体外培养的小鼠垂体分泌LH的作用。其活性依此相当对照垂体的115.4,114.2,140,160和179%。小鼠于妊振第7～9天或第1～5天,每只scphe¹⁴GRP1mg·d^-1,或于妊娠第2～4天每只scphe¹⁴GRP(5～14)1mg·d^-1,有40～60%的妊娠动物出现死胎。

Abstract: Synthetic GRP and its analogues ,GRP-NH₂,Glu^7.9.14 Lys^6.10 GRP(6～14),Phe¹⁴GRP(5～14)andPhe¹⁴GRP,at the concentration of 0.05 mmol·L^-1 were shown tohave stimulatory effect on LH secretion in cultured mouse pituitary in vitro. The luteotropin releasingactivity of GRP and its analogues was estimated to be 115.4,114.2,140.0,162.0 and 179.0%ofthe control group,respectively. Subcutaneous administration of Phe14GRP on days 7～9 and 1～5 of pregnancy and phe¹⁴GRP(5～14)on days 2～4 of gestation at dosage of 1 mg·d^-1(eachmouse)caused fetal death in 40～60% of mice.