利巴韦林注射液体内外抗流感病毒作用研究

In vitro and in vivo anti-influenza virus activity of ribavirin injection

摘要:
利巴韦林具有广谱抗病毒活性, 对多种DNA或RNA病毒体内外都有抑制作用。本文对利巴韦林注射液的体内外抗流感病毒活性进行研究, 体外实验采用细胞病变 (CPE) 法研究了利巴韦林注射液对流感病毒甲乙型的活性, 体内实验采用小鼠模型研究了利巴韦林注射液对流感病毒A/FM/1/47 (H1N1) 鼠肺适应株感染小鼠的保护作用。结果显示, 在体外实验中, 利巴韦林注射液对所检测的7种病毒株均有显著的抑制活性; 在体内实验中, 利巴韦林注射液可以显著提高感染小鼠的存活率和平均生活日, 显著改善感染小鼠的肺病变和肺指数。

Abstract:
Ribavirin is a broad-spectrum inhibitor against several unrelated DNA or RNA viruses in vitro and in vivo. In this paper the in vitro and in vivo study of anti-influenza virus activity of ribavirin (RBV) injection had been reported. The in vitro antiviral activity of ribavirin injection against influenza virus A and B was studied by CPE. The in vivo protective action of ribavirin injection against influenza A/FM/1/47(H1N1) mouse adapted strain infected mouse was studied with mouse model. The results showed ribavirin injection has strong inhibitory activity against 7 virus strains tested in vitro. Ribavirin injection could significantly increase virus infected mouse survival rate and survival days and improve lung pathogen and lung index.